Project

Part:BBa_K3113210:Design

Designed by: Sarah Brajkovic, Joshua Hesse   Group: iGEM19_Munich   (2019-10-13)


pCAG_CD63_Asn180_6xHis_Hibit_L7Ae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The His-tag was integrated into the large extracellular loop to enable purification of exosomes.


Source

CAG promoter, 6xHis: 6x repeated histidine sequence included in an extracellular loop of CD63, CD63: exosomal membrane protein, HiBit: small part of nanoluc luciferase, L7Ae: archeal ribosomal RBP


References